Enterococcus faecalis removal after rotary canal instrumentation and irrigation with 5% sodium hypochlorite and 2% chlorhexidine gluconate with/without 1,7% EDTA
Background: Enterococcus faecalis is the oral microorganism that is most related to failure of endodontic therapy and apical recurring infections. It can survive, colonize tubules and reinfect the root canal because it persists after preparation, irrigation and even obturation. Objective: Compare th...
Autor Principal: | Espinel Pinzón, Mercy Lorena; Pontificia Universidad Javeriana |
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Otros Autores: | García Romero, Diana Carolina; Pontificia universidad Javeriana, Olarte Collazos, Adriana Margarita; Pontificia Universidad Javeriana, Barajas Villamizar, Iomara; Pontificia Universidad Javeriana, Barrientos Sánchez, Silvia; Pontificia Universidad Javeriana |
Formato: | info:eu-repo/semantics/article |
Idioma: | spa |
Publicado: |
Editorial Pontificia Universidad Javeriana
2009
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Acceso en línea: |
http://revistas.javeriana.edu.co/index.php/revUnivOdontologica/article/view/663 |
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Sumario: |
Background: Enterococcus faecalis is the oral microorganism that is most related to failure of endodontic therapy and apical recurring infections. It can survive, colonize tubules and reinfect the root canal because it persists after preparation, irrigation and even obturation. Objective: Compare the effectiveness of irrigants 5% sodium hypochlorite and 2% chlorhexidine gluconate with or without the use of 1.7% EDTA as chelate. Methods: This was an in vitro experimental study in which 30 extracted premolar roots were prepared with rotary instruments. Each specimen was irrigated with one of the combinations of substances and cultured in BHI agar. A fourth group consisted of a control with saline solution. E. faecalis growth was measured through counting CFUs. Results: Uncountable CFUs were observed in all samples of the control group. There was a higher amount of CFUs in groups 3 (5% NaOCl + 1.7% EDTA) and 4 (2% Chlorhexidine gluconate + 1.7 EDTA). None of the irrigants with or without chelate completely removed E. faecalis. Conclusions: The use of 1.7 EDTA decreased the effectiveness of the irrigating substances in the removal of the microorganism. Both, sodium hypochlorite and chlorhexidine gluconate were realtively effective removing E. faecalis. |
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