Isolation and Culture of Postnatal Stem Cells from Deciduous Teeth
Background: Currently, degenerative diseases represent a public health problem; therefore, the development and implementation of strategies to fully or partially recover of damaged tissues has a special interest in the biomedical field. Therapeutic strategies based on mesenchymal stem cells transpla...
| Autor Principal: | Olávez, Daniela; Facultad de Odontología Universidad de Los Andes |
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| Otros Autores: | Salmen, Siham; Instituto de Inmunología Clínica, Universidad de Los Andes., Padrón, Karla; Facultad de Odontología. Univerisdad de Los Andes., Lobo, Carmine; Facultad de Odontología. Univerisdad de Los Andes., Díaz, Nancy; Facultad de Odontología, Universidad de Los Andes., Berrueta, Lisbeth; Doctora en Inmunología por Instituto Venezolano de Investigaciones Científicas (IVIC). Instituto de Inmunología Clínica, Facultad de Medicina, Universidad de Los Andes, Venezuela., Solorzanio, Eduvigis; Facultad de Odontología, Universidad de Los Andes. |
| Formato: | info:eu-repo/semantics/article |
| Idioma: | spa |
| Publicado: |
Editorial Pontificia Universidad Javeriana
2014
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| Acceso en línea: |
http://revistas.javeriana.edu.co/index.php/revUnivOdontologica/article/view/5870 |
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| Sumario: |
Background: Currently, degenerative diseases represent a public health problem; therefore, the development and implementation of strategies to fully or partially recover of damaged tissues has a special interest in the biomedical field. Therapeutic strategies based on mesenchymal stem cells transplantation from dental pulp have been proposed as an alternative. Purpose: To develop a mesenchymal stem cells culture isolated from dental pulp of deciduous teeth. Methods: The mesenchymal stem cells isolation was performed from dental pulp of two deciduous canines, freshly extracted with therapeutic indication. Specimens were cultured in D-MEM medium with 20% FBS at 37 °C and 5% CO2. The medium was changed every three days. 80-90% confluence was achieved after three weeks. Cells were stained with DAPI and STRO-1 and analyzed through flow cytometry and fluorescence microscopy. Results: The analysis showed that adherent cells had a fusiform fibroblast-like morphology and colony forming units (CFUs) were observed. 17% of the whole population was STRO-1+ and 26% of the larger and more complex population was positive to this antigen. Additionally, STRO-1+ cells were localized preferential in UFCs. Conclusions: The protocol described here could be used to enriching mesenchymal stem cells from dental pulp extracted from deciduous teeth and as a starting point for future therapeutic trials and their application in tissue regeneration.KEYWORDScultured cells; stem cells; deciduous teeth |
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