Extracción y purificación de la enzima monóxido de carbono deshidrogenasa a partir de la bacteria carboxidótrofa Oligotropha carboxidovorans

This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obta...

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Autor Principal: Cuervo, Raúl A.
Otros Autores: Benítez, Neyla, Hurtado, Clara, Bravo, Enrique, Torres, Walter, Larmat, Fernando
Formato: info:eu-repo/semantics/article
Idioma: spa
Publicado: Universidad de San Buenaventura - Cali 2017
Materias:
Acceso en línea: 1794-192X
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Sumario: This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obtain the crude enzymatic extracts, the crops underwent a breakdown by sonication, solvolysis with non-ionic detergent and centrifugation to eliminate the cellular debris. During the purification process, the crude enzymatic extracts were passed through a molecular exclusion column as well as through an ion-exchange one.