Extracción y purificación de la enzima monóxido de carbono deshidrogenasa a partir de la bacteria carboxidótrofa Oligotropha carboxidovorans
This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obta...
| Autor Principal: | Cuervo, Raúl A. |
|---|---|
| Otros Autores: | Benítez, Neyla, Hurtado, Clara, Bravo, Enrique, Torres, Walter, Larmat, Fernando |
| Formato: | info:eu-repo/semantics/article |
| Idioma: | spa |
| Publicado: |
Universidad de San Buenaventura - Cali
2017
|
| Materias: | |
| Acceso en línea: |
1794-192X |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: |
This paper reports the extraction and purification of the carbon monoxide dehydrogenase enzyme from the Oligotropha carboxidovorans bacteria, OM 5 strain. The bacteria grew autotrophically in a mineral medium, in carbon monoxide saturated atmosphere, at 30°C with constant agitation. In order to obtain the crude enzymatic extracts, the crops underwent a breakdown by sonication, solvolysis with non-ionic detergent and centrifugation to eliminate the cellular debris. During the purification process, the crude enzymatic extracts were passed through a molecular exclusion column as well as through an ion-exchange one. |
|---|