Análisis de los cromosomas mitóticos de seis diferentes morfotipos de pristimantis achatinus (anura: craugastoridae) de una población al noroccidente del distrito metropolitano de Quito “Las Tolas”
In Ecuador, there is great diversity of frogs, gender Pristimantis this is the most numerous, there are cases where members of the same species have morphological variations which hinder their identification, so that the use of other techniques such as required cytogenetics, for proper characterizat...
| Autor Principal: | Carrera Valencia, Luis Alejandro |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/8857 |
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| Sumario: |
In Ecuador, there is great diversity of frogs, gender Pristimantis this is the most numerous, there are cases where members of the same species have morphological variations which hinder their identification, so that the use of other techniques such as required cytogenetics, for proper characterization. An example is the case of Pristimantis achatinus, species of which six morphotypes were recorded in the town of Las Tolas, in the province of Pichincha. This paper aims to analyze mitotic chromosomes six morphotypes, determining each number, morphology and relative length by measuring techniques and chromosome banding C, DAPI and NOR. For this purpose 10 metaphases were analyzed for each morphotype from duodenal epithelium. Members analyzed Pristimantis achatinus presented 2n=36 chromosomes, all telocentric in both males and females. In five of the six morphotypes bands C were detected exclusively in the centromeric region, while in the sixth morph red five bands C telomeric were found in the long arm (q). The Nucleolar Organizer Region, NOR, was located on chromosome pair 15 in all morphotypes associated with a secondary constriction. The DAPI bands, labeled strong and exclusively in the centromeric region of all chromosome pairs, all morphotypes. With these results we can say that the red morph differs from the others by the presence of bands C telomeric, which could suggest the existence of some chromosomal phenomenon that may lead these individuals enter into a process of speciation. |
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