Western Blot como método diagnóstico de la hidatidosis humana, utilizando fluido del metacestodo de Echinococcus sp.
Hydatidosis or echinococcosis is a disease of worldwide distribution produced by a parasitic flatworm of the genus Echinococcus. The larval stage of the cestode develops cysts in liver and lung tissue of cattle and humans accidentally infected. The developed hydatides cause compression to surroundin...
| Autor Principal: | Ramos Sarmiento, Daniel Alexander |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15339 |
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| Sumario: |
Hydatidosis or echinococcosis is a disease of worldwide distribution produced by a parasitic flatworm of the genus Echinococcus. The larval stage of the cestode develops cysts in liver and lung tissue of cattle and humans accidentally infected. The developed hydatides cause compression to surrounding organs, anaphylaxis and death in patients. It is diagnosed by combining radioimage methods and immunoassays, obtaining unreliable leads. Thus, we intend to develop the Western Blot technique as a diagnostic method of human hydatidosis, using Echinococcus sp. Metacestodo fluid, as a complementary assay for enzyme-linked immunosorbent assay (ELISA).
Firstly, by means of morphometric evaluation of protoescolex rostellar hooks present in the solid fraction of hydatid fluid of cysts rooted in the parenchyma of porcine liver tissue, it was possible to identify the species Echinococcus granulosus, the cause of the parasitic zoonosis known as unilocular hydatidosis.
By means of the preliminary screening analysis by the ELISA Indirect Home Project 2-program PROPAD, 40 blood samples belonging to the seroteca of the same program were selected. Again, the sera were screened by the same technique and later evaluated by the NovaTec NovaLisa Echinococcus IgG ELISA Commercial Kit, obtaining 31 sera with negative reaction and 9 samples with positive reaction for human hydatidosis. The totality of the blood samples were evaluated by Western Blot qualitatively, corroborating with the same derivations as the aforementioned ELISA tests, for which the technique obtained a sensitivity and specificity of 100% being reliable for the diagnosis. |
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