Evaluación de la eficacia de Trichoderma sp y Pseudomona sp para biorremediación de suelos contaminados con hidrocarburos
The aim of this research was evaluate the power of bioremediation of the fungus Trichoderma sp and the bacteria Pseudomonas fluorescens in a sample of soil contaminated by hydrocarbons, in order to reduce the TPH (total polycyclic hydrocarbons) in soil. This project was completed in two phases...
| Autor Principal: | Muñoz Cuaical, Stalin David |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13228 |
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| Sumario: |
The aim of this research was evaluate the power of bioremediation of the fungus
Trichoderma sp and the bacteria Pseudomonas fluorescens in a sample of soil
contaminated by hydrocarbons, in order to reduce the TPH (total polycyclic
hydrocarbons) in soil. This project was completed in two phases: the field work
consisted of soil sampling and the laboratory work consisted of installation of an assay
with a DBCA system with 4 treatments (T0: control soil, T1: Soil + Trichoderma sp,
T2: Soil + Pseudomonas fluorescens and T3 : Soil with Pseudomonas fluorescens +
Trichoderma sp ) and 4 repetitions with controlled environmental conditions such as
pH, temperature, electrical conductivity and moisture; the physico-chemical and TPH
analyzes was performed at beginning and end of the assay for 3 months.
The soil at beginning of the assay had macro and micro elements which are essential
for microorganisms to degrade hydrocarbons. The initial TPH contamination in soil
was 42070.3 mg / kg, and at the end of the assay the T1, T2 and T3 obtained an
effective remediation, settling down within the permissible limits of RAOHE
(environmental regulations hydrocarbon activities); demonstrating that all treatments
work independently and in a consortium. The T3 (consortium) was the treatment that
degraded more amount of hydrocarbons with a value of 379.2 mg / kg, the reason was
because both microorganisms had the necessary mechanisms to degrade the
hydrocarbon molecules, showing that bioremediation is an ecological and effective
process for the degradation of hydrocarbons. |
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