Análisis filogenéntico de quinua silvestre (Chenopodium quinoa Willd) utilizando el gen rpoB en muestras provenientes de las provincias de Carchi, Imbabura, Cotopaxi y Chimborazo
The willd quinoa (Chenopodium quinoa Willd.) is one of the most important species in Ecuador for being a resource of our own genetic Ecuadorian highlands. The distribution areas of this Amaranthacea covers the vast majority of the Andean region of our country, being the provinces of Carchi, Imbabura...
| Autor Principal: | Navarrete Verdesoto, Stephany Johanna |
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| Otros Autores: | Suasnavas Chacón, Byron Alejandro |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/9408 |
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| Sumario: |
The willd quinoa (Chenopodium quinoa Willd.) is one of the most important species in Ecuador for being a resource of our own genetic Ecuadorian highlands. The distribution areas of this Amaranthacea covers the vast majority of the Andean region of our country, being the provinces of Carchi, Imbabura, Cotopaxi and Chimborazo the most important, therefore in this study the sampling of 8 individuals is by locality being targeted areas: El Hato (Carchi), Los Olivos (Imbabura), Belisario Quevedo (Cotopaxi) and Colta (Chimborazo), all of these samples were used for DNA genotyping, in order to establish their phylogenetic relationships The extraction and purification of DNA was performed by the method proposed by Doyle and Doyle (1987) modified protocol. Genotyping was accomplished by DNA amplification of the rpoB gene fragment of 456 bp amplicons obtained later in the ABI PRISM 3730XL equipment Macrogen Analyzer sequenced. The results of genetic analysis allowed the construction of phylogenetic trees which revealed that genetic diversity is extremely high for a population-way inter and intra-population variability obtaining a percentage of 77, 4% for individuals from the provinces of Carchi and Imbabura and 70.2% for individuals from the provinces of Cotopaxi and Chimborazo, these data were corroborated with the values obtained by statistical tests of the AMOVA and genetic distance; the statistical significance of AMOVA hinted that the similarity between individuals of the northern provinces (Carchi and Imbabura) is only 15%, whereas in the central provinces (Cotopaxi and Chimborazo) is 25%. |
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