Determinación de residuos de antibióticos en carne y vísceras de origen bovino que se expenden en la ciudad de Quito.
The objective of this research was to detect and quantify the concentrations of residues of penicillin G, sulfonamides, streptomycin, gentamicin, oxytetracycline, tylosin and enrofloxacin in samples of muscle, liver and kidney bovine. Seven antibiotics were analyzed in 27 samples, which were taken f...
| Autor Principal: | Noroña Bastidas, Gabriel Alejandro |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/14502 |
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| Sumario: |
The objective of this research was to detect and quantify the concentrations of residues of penicillin G, sulfonamides, streptomycin, gentamicin, oxytetracycline, tylosin and enrofloxacin in samples of muscle, liver and kidney bovine. Seven antibiotics were analyzed in 27 samples, which were taken from five markets and four supermarkets in Quito city. The samples were identified and transported for analysis to the laboratorio de Contaminantes de Productos Pecuarios de AGROCALIDAD. For the determination of antibiotic residues, BIOO Scientific test kits based on the enzyme linked immunosorbent assay (ELISA) method, which consists of a competitive colorimetric assay, in which the antibody from a sample competes with the Conjugated by antigen binding sites. The results determined that 22 of the 27 samples were positive to penicillin residues, 12 to sulfonamide residues, 5 to oxytetracycline residues; 3 to gentamicin and tylosin residues; 2 to streptomycin residues and absence to residues of enrofloxacin. The family of antibiotics that presented the highest concentration of residues was aminoglycoside. In order to establish the levels of acceptance of the samples evaluated and to know if the concentrations are within the Maximum Residue Limits (MRLs) of veterinary drugs in muscle, liver and kidney, were taken as references to the MRLs established by the Codex Alimentarius Commission and by Regulation 37/2010 of the European Union. Of the positive samples, none exceeded the MRLs allowed by the international food standard. |
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