An approach for micropropagation of blueberry (Vaccinium corymbosum L.) plants mediated by temporary immersion bioreactors (TIBs)
A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and Brillita. Plant cultures were developed in a controlled environment with 0.4...
| Autor Principal: | Arencibia-Rodríguez, Ariel |
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| Otros Autores: | Vergara, Carolina, Quiroz, Karla, Carrasco, Basilio, Bravo, Carmen, García-González, Rolando |
| Formato: | Artículo |
| Idioma: | English |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://repositorio.ucm.cl:8080/handle/ucm/1230 |
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| Sumario: |
A new procedure for blueberry (Vaccinium corymbosum L.) micropropagation in programmed Temporary Immersion
Bioreactors (TIBs based on two separate bottles) was developed for the commercial genotypes Biloxi, Sharp Blue and
Brillita. Plant cultures were developed in a controlled environment with 0.4 MPa CO2 enrichment, sucrose-reduced
medium, and light intensity of 60 M m−2 ·s−1. Principal component analysis showed that component 1 (C1) grouped
64.08% of the total variability, while the first two components accounted for 86.97%. Representation of the principal
components demonstrated three clusters corresponding with the blueberry genotypes, and within each cluster plants
micropropagated in agar-base medium grouped separately from those plants multiplied in TIBs. Both plant number and
total internodes traits (related to the productive efficiency) were demonstrated superior in blueberries propagated in
TIBs. Additionally, when transferred to greenhouse conditions, blueberries propagated in TIBs showed higher adaptability
and growing rates than those cultured by the conventional approach, altogether evidencing the occurrence of a
photomixotrophic stage in the vitroplantlets cultured in TIBs. |
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