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Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile

A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce highquality DNA with yields sufficient to allow its use i...

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Autor Principal: Cáceres-Ruz, Pablo
Otros Autores: Cordero, Cecilia, González-Silva, Gloria, Quiroz, Karla, Bobadilla, Juan, Bravo-Castillo, Carmen, Caligari, Peter D. S., Carrasco, Basilio, García-González, Rolando
Formato: Artículo
Idioma: English
Publicado: 2017
Materias:
Hydrophilic forests
16S ribosomal RNA
PCR
Rhizosphere
Soil DNA extraction
Acceso en línea: http://repositorio.ucm.cl:8080/handle/ucm/1269
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