Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera
Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Cha...
| Autor Principal: | Villadiego Marmolejo, Luisa Fernanda |
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| Otros Autores: | Villarreal Buitrago, Laura Y., Richtzenhain, Leonardo J., Brandão, Paulo E. |
| Formato: | info:eu-repo/semantics/article |
| Idioma: | spa |
| Publicado: |
Universidad de La Salle. Revistas. Revista de Medicina Veterinaria.
2007
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| Materias: | |
| Acceso en línea: |
http://revistas.lasalle.edu.co/index.php/mv/article/view/1798 |
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| Sumario: |
Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Chain Reaction (PCR) technique to detect PCV-2 in swine serum and to apply the validated technique in swine serum samples to detect PCV-2. After the application of two different PCRs, 100% of the surveyed animals were negative to PCV-2; furthermore, the PCR targeted to a region between ORFs 1 and 2 of the virus was found more sensitive when compared to another PCR targeted to the capsid protein gene. As a conclusion, PCR is a valid technique to detect PCV-2 in swine serum and the surveyed population was free of the virus. |
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