CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SD...
| Autor Principal: | Rodríguez Sáenz, Anayervid; Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana |
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| Otros Autores: | Acosta Losada, Orlando; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá, Peñaranda, José; Unidad de Bioquímica Instituto de Biotecnología Universidad Nacional de Colombia, Bogotá |
| Formato: | info:eu-repo/semantics/article |
| Idioma: | eng |
| Publicado: |
Pontificia Universidad Javeriana
2013
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| Materias: | |
| Acceso en línea: |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4821 |
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| Sumario: |
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles. |
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