PRODUCCIÓN DE LA PROTEÍNA RECOMBINANTE HUMANA HSC70 Y SU APLICACIÓN EN EL DESARROLLO DE UN SISTEMA DE DETECCIÓN POR ELISA
In order to understand how the Heat Shock Proteins (HSPs) are involved in antitumoral immunity activation process, through their release from tumoral cells and their interaction with dendritic cells (DC), it is necessary to develop the proper tools for research on those processes. Therefore, our m...
| Autor Principal: | Espejo Mojica, Ángela Johana; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá |
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| Otros Autores: | Sosa Molano, Ángela Catalina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Carrascal Camacho, Ana Karina; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Cifuentes Rojas, Catherine; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Barreto, Alfonso; Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá |
| Formato: | info:eu-repo/semantics/article |
| Idioma: | eng |
| Publicado: |
Pontificia Universidad Javeriana
2004
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| Materias: | |
| Acceso en línea: |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5036 |
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| Sumario: |
In order to understand how the Heat Shock Proteins (HSPs) are involved in antitumoral immunity activation process, through their release from tumoral cells and their interaction with dendritic cells (DC), it is necessary to develop the proper tools for research on those processes. Therefore, our main aim was to produce and purify the recombinant human protein Hsc70 (rh-Hsc70) obtained from E. coli BL21DE3pLysS pET28a(+)/Hsc70 strain and in that way, it can be used at in-vitro studies of its biological functions over DC. On the other hand, an ELISA system was established for detecting and quantifying the Hsc70 in soluble form |
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