Diversidad de hongos asociados a los intestinos de escarabajos (Passalidae) que habitan en el Parque Nacional Yasuní y evaluación de degradación de celulosa in vitro
The decomposition of soil organic matter (SOM) in the forest is essential for the nutrient cycling in edaphic ecosystems. In this processes are not only involved abiotic components, but also biotic such as microorganisms and soil invertebrates. Passalidae is a family of the order Coleoptera that pos...
| Autor Principal: | López Chávez, Sofía Alejandra |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
PUCE
2016
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| Materias: | |
| Acceso en línea: |
http://repositorio.puce.edu.ec/handle/22000/10383 |
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| Sumario: |
The decomposition of soil organic matter (SOM) in the forest is essential for the nutrient cycling in edaphic ecosystems. In this processes are not only involved abiotic components, but also biotic such as microorganisms and soil invertebrates. Passalidae is a family of the order Coleoptera that posses saprophytic habits and requires gut microbes to contribute wood’s degradation and digestion. Adults of three Passalidae species were collected in Yasuni National Park of Ecuador. Filamentous fungi were isolated from intestinal tracts of adult beetles and identified sequencing the internal transcribed spacer (ITS) region and analyzed with phylogenetic tree construction. The fungal isolates were assigned to 2 phyla, 5 orders, 8 families and 11 genera and 19 species. Trichoderma was the most abundant genus detected in all Passalidae species and at all sites sampled. The enzymatic capacity to digest cellulose was positive for extracts of beetle guts and for all fungi tested. Highest scores in these assays were achieved by Clonostachys rossmaniae and Epicoccum nigrum fungal isolates. Diversity and composition analyses of fungal species demonstrated that Passalidae beetles harbor an important diversity not only of fungal number species but phylogenetic composition, and a complex fungal dynamic. Fungal communities structure depends on Passalidae species host and surrounding environment. The current research provides new evidence of possible endosymbiotic relationships that several types of microbes are able to establish with wood-feeding beetles, which may play an important role to beetles nourishment during cellulose digestion and fermentation. |
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