Pruebas bajo invernadero de cepas de Bacillus subtilis como agente de biocontrol de Alternaria spp. en Brassica oleracea var italica y técnicas de conservación de cepas
Broccoli is considered one of the most important vegetables of Ecuador in the national and international market, however the percentage of the culture of broccoli for export is low due to the presence of diseases caused by fungi, mainly by the genus Alternaria characterized by being manifested...
| Autor Principal: | Caicedo Morales, Sugey Estefania |
|---|---|
| Otros Autores: | Chacón Muela, Jessica Alexandr |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13545 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: |
Broccoli is considered one of the most important vegetables of Ecuador in the national
and international market, however the percentage of the culture of broccoli for export is
low due to the presence of diseases caused by fungi, mainly by the genus Alternaria
characterized by being manifested in mature leaves, stems and flower heads causing
significant economic losses.
The assay was carried out in two phases; in the laboratory phase, both Alternaria spp.
and Bacillus subtilis were propagated and the strains were conserved. And in the field
phase the application of the pathogen inoculum and the biocontrolator. The antagonistic
capacity of Bacillus subtilis was evaluated using a severity scale designed as a function
of the number of lesions caused by the fungus and an ABCPE (Area under the disease
progress curve) was performed, that indicated that there is a difference in at least one
treatment and statistical differences among the hybrid varieties. Also a post-harvest
analysis was performed by a Tukey test at 5%, obtaining the percentage of severity in
the harvested flower heads, being the treatment IB6; Isolated from the province of
Imbabura, the best strain with antagonistic capacity and the Avenger variety which
presented a lower incidence of the disease compared to the variety Domador.
The method used for conservation and the best results that were produced in the
reactivation was the Cryobank, since it provided a culture without contamination and
with a high percentage of recovery |
|---|