Evaluación de la eficiencia fotosintética en el cultivo in vitro de Brassica oleracea var. Italica.
The measurement of the photosynthetic efficiency in the in vitro culture presents multiple applications, such as the regeneration and rapid propagation of healthy whole plants, a factor directly related to low levels of stress. The present work sought to evaluate the concentration of M&S salts...
| Autor Principal: | Masache Almeida, Evelin Nathaly |
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| Otros Autores: | Meneses Morillo, Johana Gabriela |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15692 |
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| Sumario: |
The measurement of the photosynthetic efficiency in the in vitro culture presents multiple applications, such as the regeneration and rapid propagation of healthy whole plants, a factor directly related to low levels of stress.
The present work sought to evaluate the concentration of M&S salts to determine the optimum medium in vitro introduction and multiplication, to evaluate two substrates for acclimatization and to analyze the photosynthetic efficiency in the multiplication and acclimatization phases. For the evaluation of M&S salts, the three concentrations M1 (100 %), M2 (50 %) and M3 (25 %) were optimal for germination during in vitro introduction, while M1 and M2 were adequate during multiplication, obtaining 94 at 100 % budding and a greater length in the seedlings in a range of 3,39 to 4,54 cm; for the evaluation of substrates, the S2 substrate containing peat and humus (3:1) was optimal, with 92 % of rooted plants. For the analysis of the photosynthetic efficiency the concentration of M1 salts, resulted with the lowest level of stress (0,71 to 0,73), finally the substrate S2 presented the highest photosynthetic efficiency in acclimation (0,72) allowing to obtain vitroplants pathogen-free with good health status, low stress levels and high fluorescence emission. |
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