Evaluación in vitro de la capacidad bacteriana para remover cadmio en aguas residuales sintéticas

Accumulation and presence of heavy metals such as cadmium in wastewater cause concern because of its high toxicity and complexity of its elimination. One of the methods for metal removal is the use of bacteria; some species can accumulate metals or convert them into less toxic forms, due to their...

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Autor Principal: Rubio Caiza, Carmen Lucrecia
Otros Autores: Terán Moreno, Katherin Lisvet
Formato: bachelorThesis
Idioma: spa
Publicado: 2016
Materias:
Acceso en línea: http://dspace.ups.edu.ec/handle/123456789/13070
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Sumario: Accumulation and presence of heavy metals such as cadmium in wastewater cause concern because of its high toxicity and complexity of its elimination. One of the methods for metal removal is the use of bacteria; some species can accumulate metals or convert them into less toxic forms, due to their characteristics or morphology. The aim of this research was to evaluate in vitro the capacity of bacteria to remove cadmium in synthetic wastewater. For the experimental phase, bacteria were isolated and then biochemical tests to identify them were performed. Five species were selected: Acinetobacter sp, Pseudomona sp (Morfología fenotípica 1), Pseudomona sp (Morfología fenotípica 2), Aeromona hydrophila and Aeromona sp, which they were cultivated in liquid medium at concentrations of 2 ppm and 10 ppm of cadmium. Metal removal was evaluated each two hours by measurement of the variables temperature, pH and turbidity at room temperature and controlled temperature (25°C). Concerning data analysis, the experimental design was applied by randomized block analysis and statistical test: Shapiro-Wilk, Mann Whitney U and T Student. At the end of the experiment, the 10 ppm's concentration of cadmium inhibited the growth of all species, however, at 2 ppm the bacteria were able to adapt to the environment. Acinetobacter sp presented 77.49% removal of cadmium, Pseudomonas sp. (Phenotypic Morphology 1) presented 68.78 % and Aeromona hydrophila indicated 66.98% at 2 ppm’s concentration.