Evaluación de la biodegradación de suelos contaminados con hidrocarburos utilizando Aspergillus niger, Pleurotus ostreatus y Pseudomonas aeruginosa
The use of natural resources such as hydrocarbons has generated pollution and environmental impact in the Ecuadorian Amazon. This research aims to evaluate the biodegradation capacity of Aspergillus niger, Pleurotus ostreatus and Pseudomonas aeruginosa in soils contaminated with TPH's (Total Pe...
| Autor Principal: | Cevallos Paguay, Tanya Carolina |
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| Otros Autores: | García Díaz, Jonathan Darío |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15681 |
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| Sumario: |
The use of natural resources such as hydrocarbons has generated pollution and environmental impact in the Ecuadorian Amazon. This research aims to evaluate the biodegradation capacity of Aspergillus niger, Pleurotus ostreatus and Pseudomonas aeruginosa in soils contaminated with TPH's (Total Petroleum Hydrocarbons) and HAP´s (Polycyclic Aromatic Hydrocarbons).
In this project soil from the city of Francisco de Orellana (El Coca) was sampled, which was contaminated with grade 28 API hydrocarbon, after which the physicochemical analysis, quantification of TPH's and HAP's were carried out; besides isolating and identifying the microorganisms of the soil. With this characterization the biodegradation capacity of Aspergillus niger, Pleurotus ostreatus and Pseudomonas aeruginosa was evaluated.
A DBCA (randomized complete block design) of 6 treatments was used. Twice a week, 100 mL of inoculum was applied at a concentration of 108 CFU / mL, temperature, humidity, pH and electrical conductivity were measured; with turn of the ground. The total duration of the experiment was three months.
At the end of the investigation, biodegradation percentages ranging between 44 % and 10 % were obtained, due to the enzymatic complex present in the microorganisms Aspergillus niger, Pleurotus ostreatus and Pseudomonas aeruginosa, the latter being the best biodegradator for this project, possibly due to having biosurfactants, monooxygenases and dioxygenases that contributed to the transformation of recalcitrant compounds, demonstrating that bioaugmentation is an alternative for the recovery of soils contaminated with hydrocarbons. |
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