Identificación morfológica y molecular de Plutella xylostella y sus parasitoides en cultivos de brasicáceas de la región sierra del Ecuador

Plutella xylostella L., also known as Diamondback moth, cruciferous moth (Brassicaceae) or coles moth is a plague of great importance worldwide. Ecuador is no stranger to the attack of this lepidopteran; despite of that there are no studies tending to know which are the natural enemies that attack t...

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Autor Principal: Gavilanes Córdova, Kimberly Gabriela
Formato: bachelorThesis
Idioma: spa
Publicado: 2018
Materias:
Acceso en línea: http://dspace.ups.edu.ec/handle/123456789/15819
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Sumario: Plutella xylostella L., also known as Diamondback moth, cruciferous moth (Brassicaceae) or coles moth is a plague of great importance worldwide. Ecuador is no stranger to the attack of this lepidopteran; despite of that there are no studies tending to know which are the natural enemies that attack this plague, that is why we propose to do the present investigation, the objective was to identify P. xylostella and its parasitoids through the use of taxonomic keys and molecular techniques as a contribution to the biological control of pests in the Brasicaceas crops. The identification of both the pest and its parasitoid was done by means of taxonomic characters, then: DNA was extracted from the specimens, the polymerase chain reaction, sequencing using the Sanger method, and finally the identification of the species in question. It was determined that the plague that attacks Brassicaceae crops in Ecuador is P. xylostella. Its parasitoid in the provinces of Pichincha and Cotopaxi