Definición de protocolos para el uso de fitohormonas en el crecimiento de orquídeas a nivel in vitro.
In this research protocols for the use of gibberellic acid (GA3), butyric indole acid (IBA) and kinetin (Kin) were established through in vitro culture of Epidendrum jamiesonis, Epidendrum schistochilum and Oncidium ornithorhynchum, native orchids of Ecuador. Firstly, ideal reference concentra...
| Autor Principal: | Aucapiña Criollo, Cristina Belén |
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| Otros Autores: | López Peña, Paúl Alexander |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12147 |
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| Sumario: |
In this research protocols for the use of gibberellic acid (GA3), butyric indole acid (IBA)
and kinetin (Kin) were established through in vitro culture of Epidendrum jamiesonis,
Epidendrum schistochilum and Oncidium ornithorhynchum, native orchids of Ecuador.
Firstly, ideal reference concentrations were calibrated by bioassay using Solanum
lycopersicum. To establish a dynamic in the percentage of germination by adding
gibberellins, orchid seeds were sown in the Knudson C Modified Medium Plus and 130
µL of each hormone solution were added, determining the ideal concentration of 0,1 mg/L
for Epidendrum and 0,2 mg/L for Oncidium. In addition, the effect of IBA, Kin and the
combination IBA x Kin was analyzed in protocorms of the species Epidendrum jamiesonis
and Epidendrum schistochilum. These were planted in the Orchid Maintanance Medium
and 130µL of hormone solution was added. Regarding the kinetin, an ideal concentration
of 0,01 mg/L for shoot length and 0,4 mg/L for number of shoot were established. It was
also determined that the point of auxin-cytokinin balance in Epidendrum schistochilum is
7,5 mg/L IBA x 0,1 mg/L Kin, but in Epidendrum jamiesonis is 3 mg/L IBA x 0,4 mg/L
Kin. However, a better development in the number of shoot, root and leaf growth was
observed when applying the combination of hormones solution than individually. |
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