Identificación molecular de especies de orquídeas del género Dracula, mediante el sistema BARCODE
The family (Orchidaceae) stands out as one of the most important because it contains a large number of species of high economic value; At present, within the Dracula genus around 130 species are recognized in the world and 56 species are described in Ecuador, a total of 144 individuals of Dracula we...
| Autor Principal: | Iza Arteaga, Mario León |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15043 |
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| Sumario: |
The family (Orchidaceae) stands out as one of the most important because it contains a large number of species of high economic value; At present, within the Dracula genus around 130 species are recognized in the world and 56 species are described in Ecuador, a total of 144 individuals of Dracula were collected in different orchids within the provinces of Pichincha and Carchi, molecular identification through the BARCODE system to determine the possible existence of new species and the resolution of subgeneric problems of this genus. The absence of flowering for its morphological identification makes use of molecular techniques that allow its effective identification. We proceeded to the extraction of DNA from the species collected, then the chloroplastic regions matK, rpoC1 and rpoB were amplified using the conventional PCR technique for sequencing and finally the phylogeny of the genus was established by the MEGA 6 software. The results obtained showed that the matK region presents a good potential for discrimination up to the subsection level, the rpoC1 and rpoB markers did not prove useful for this study. Therefore it is suggested to investigate with new regions of chloroplastic and nuclear DNA that allow to solve the subgeneric inconsistencies of the genus Dracula. |
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