Identificación molecular de las especies del género Brugmansia (Solanaceae), presentes en la zona norte de los Andes del Ecuador
The Brugmansia species possess toxic alkaloid substances that are used to make psychotropic substances. Its ingestion in humans and animals is fatal in excessive doses. In Ecuador 7 species are known, of which 5 have been registered in the Andean zone, the species are: Brugmansia arborea, B. aurea,...
| Autor Principal: | Escobar Mena, Edison David |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15451 |
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| Sumario: |
The Brugmansia species possess toxic alkaloid substances that are used to make psychotropic substances. Its ingestion in humans and animals is fatal in excessive doses. In Ecuador 7 species are known, of which 5 have been registered in the Andean zone, the species are: Brugmansia arborea, B. aurea, B. x candida, B. suaveolens, B. insignis, B. versicolor, B. sanguinea and B. vulcanicola. Samples were collected in several provinces of Ecuador: Carchi, Pichincha, Cotopaxi, Napo and Morona Santiago. Obtaining 40 samples of different specimens of the genus, to which DNA was extracted by the modified Doyle & Doyle method, later amplified segments of the matK, rpoB and rpoC1 regions, by means of the conventional PCR technique, then the products were sequenced through of the Sanger technique and analyzed with the bionformatic tools: Boldsystems and Blast to verify the identity of the species. 4 phylogenetic trees were developed, 1 for each region and 1 tree concatenated the 3 regions, for this the Neighbor Joining Tree method of Mega 7 software was used; the matK region allowed the elaboration of a tree that shows the grouping of species, this in accordance with the geographical distribution of the same. The rpoB region showed less difference of groups demonstrating little variability of the gene. Additionally, the geographical distribution of the Brugmansia genus in Ecuador was studied, determining that the diversification point of the species is located in the north-eastern zone of the Andes. |
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