Cultivo de la microalga Coenochloris sp. con fracción soluble de papa (Solanum phureja L) para la obtención de pigmentos (CAROTENOIDES).
Culture of microalgae has become important due to its growth efficacy and metabolite yield. Carotenoids are photosynthetic pigments that have an antioxidant capacity and are essential for the human organism (FAO, 2002), (SEMPLADES, 2009). Coenochloris sp., is a clorophyte microalga capable of...
| Autor Principal: | Rodríguez Iza, Emilia Lucía |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12561 |
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| Sumario: |
Culture of microalgae has become important due to its growth efficacy and metabolite
yield. Carotenoids are photosynthetic pigments that have an antioxidant capacity and
are essential for the human organism (FAO, 2002), (SEMPLADES, 2009).
Coenochloris sp., is a clorophyte microalga capable of accumulating photosynthetic
pigments in optimal growth conditions. The assessment of the microalga to produce
pigments is important to categorize it as an alternative species that provides output in
the production of pigments. This research aimed to separate and quantify
photosynthetic pigments (carotenoids) from the microalgae culture Coenochloris sp.,
in mixotrophic conditions. It was feasible to adapt the microalga in laboratory
conditions in an autotrophic culture with foliar nitrophoska (3mL/L). The microalga
was assessed through three different cultures, autotrophic (CNFK), mixotrophic
(FSPGSC M) and discontinuous fed culture (FSPGSC CDA), where 2,5 mL/L of
soluble fraction of germinated peeled potato (FSPGSC) 5% were added to the
mixotrophic treatments and the discontinuous fed culture. The length of the assessment
was 29 days, where CNFK revealed the biggest concentration of carotenoids, followed
by FSPGSC CDA and FSPGSC M showing 0,4853 nm, 0,4792 nm and 0,4681
respectively. The most effective solvent to get the pigments was the DMSO. The
separation of carotenoids was more efficient by column chromatography displaying
54% of efficiency. The results obtained by HPLC have not shown presence of
carotenoids in any sample as they have been highly oxidized. |
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