Cultivo discontinuo de la microalga Coenochloris sp. con fracción soluble de papa (Solanum phureja) en condiciones mixotróficas.
The cultivation of microalgae is a biotechnological alternative due to its greater efficiency in the production of biomass in relation to autotrophic and heterotrophic cultures. The growth and quality of the biomass of the Coenochloris sp. microalgae was studied and it was cultured in a medium...
| Autor Principal: | Verdugo Castro, Karla Beatriz |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13226 |
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| Sumario: |
The cultivation of microalgae is a biotechnological alternative due to its greater efficiency
in the production of biomass in relation to autotrophic and heterotrophic cultures. The
growth and quality of the biomass of the Coenochloris sp. microalgae was studied and it
was cultured in a medium control with NOTROFOSKA fertilizer, a medium potato
soluble fraction (Solanum phureja) and a medium potato soluble fraction and
NITROFOSKA. Some tests were made in fed batch cultures (CDA) that had an initial
concentration of 1x106cel/mL (constant shake at 21.5±2°C for 20 days). A potato soluble
fraction was used in the first trial: sprout skin (FSPGCC) and without skin (FSPGSC), not
sprout with skin (FSPNGCC) and without skin (FSPNGSC); Coenochloris sp. has reached
the highest growing in FSPGSC and it got a concentration of 12.54x106cel/mL. The
growing of FSPGSC was compared related to control. A higher cellular density of
35.12x106cel/mL (1.71 mg/mL of dry biomass) was produced with FSPNGSC with
NITROFOSKA (FSPNGSC-CDA) and it surpassed the control that showed a
concentration of 26.72x106 cel/mL. The obtained meaningful statistics was 0.479 showing
the difference in cellular density determined by treatments. The proximal analysis
revealed a protein concentration of 42.00% inside control and 33.89% in FSPNGSC-CDA.
The fat value for control and FSPNGSC-CDA was 2,40% and 7,95% respectively.
These results prove that Coenochloris sp. cultured in mixotrophic conditions with potato
soluble fraction is an alternative for organic substratum in order to produce biomass with
a high protein concentration. |
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