Evaluación de la actividad antioxidante bioautográfica de cinco variedades de aceites esenciales andinos (Aristeguietia glutinosa; Myrcianthes rhopaloides; Ambrosia arborescens; Lantana camara; Minthostachys mollis)
This experimental work had the goal of assessing the antioxidant capabilities of five essential oils: Minthostachys mollis, Aristeguietia glutinosa, Ambrosia arborescens, Myrcianthes rhopaloides and Lantana camara; all of which were obtained from the Ecuadorian Andes. This work used the DPPH a...
| Autor Principal: | Guerrero Toapanta, Priscila Pamela |
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| Otros Autores: | Pozo Morales, Karla Natalia |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12184 |
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| Sumario: |
This experimental work had the goal of assessing the antioxidant capabilities of five
essential oils: Minthostachys mollis, Aristeguietia glutinosa, Ambrosia arborescens,
Myrcianthes rhopaloides and Lantana camara; all of which were obtained from the
Ecuadorian Andes. This work used the DPPH and ABTS methods to assess free
radical-capturing capacity, and it used the β-carotene Test to determine the antioxidant
activity of each essential oil. The natural referent used herein was Thymus vulgaris
essential oil, and the positive control was BHA.
According to the results obtained using the DPPH, ABTS and β-carotene methods, it
was found that the best essential oils in terms of free-radical-capturing and antioxidant
activity were Minthostachys mollis (IC50 DPPH 2,830µL/mL; IC50 ABTS
0,205µL/mL; and IC50 AA 0,119µL/mL) and Aristeguietia glutinosa (IC50 DPPH
12,972µL/mL; IC50 ABTS 0,321µL/mL; and IC50 AA 0,062µL/mL); whereas the
rest of the essential oils showed low antioxidant activity. Further, using the Kruskal
Wallis non-parametric test for DPPH and ABTS determined that there is no similarity
between the essential oils’ and the natural reference’s IC50s; however, the values most
approximate to the natural reference were those of Minthostachys mollis and
Aristeguietia glutinosa. In order to determine the molecules responsible for antioxidant
activity, this work used the DPPH bioautographic method, and chemical identities
were revealed through a Gas Chromatography/Mass Spectrometry (GC/MS) analysis.
The molecules responsible for Minthostachys mollis antioxidant activity were thymol
acetate and carvacrol acetate, whereas the molecule responsible for Aristeguietia
glutinosa activity was muurolene |
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