Desarrollo de un Protocolo de propagación in vitro de Geranium chilloense Wild. ex Kuth. Y Lupinus pubescens Benth. Para la obtención de plantas completas, para la primera etapa de restauración de las quebradas de Quito
Geranium chilloense Willd. ex Kunth known as geranio de los Chillos, is an ornamental plant, native of Los Andes it can be found in a wild way in the ravines of the Metropolitan District of Quito and it is part of the history of the native flora of Quito since has been described from the Alexander H...
| Autor Principal: | Benavides Silva, Thaly Gabriela |
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| Otros Autores: | Córdova Muñoz, Adriana Isabel |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/9372 |
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| Sumario: |
Geranium chilloense Willd. ex Kunth known as geranio de los Chillos, is an ornamental plant, native of Los Andes it can be found in a wild way in the ravines of the Metropolitan District of Quito and it is part of the history of the native flora of Quito since has been described from the Alexander Humboldt and Aimé Bonpland expedition in 1802. Lupinus pubescens Benth, also known as Ashpa chocho or Allpa chocho, is an herbaceous, native and ornamental plant due to the beauty of his flowers, declared as one of the Seven Species emblem of the flora of Quito. The present study was realized by the intention of developing a protocol for the propagation in vitro of Geranium chilloense and Lupinus pubescens to obtain complete plants. In Geranium chilloense, the best scarification consisted of three days of hydration followed by a two-hour immersion in a solution of acetic acid 10% + 90 % sterile distilled water, obtaining a higher germination percentage (31%). It was determined that P1 protocol that is based on immersed in alcohol at 96% (10 sec), then in hypochlorite sodium at 50% v/v (3% p.a), to achieve a zero of bacterial contamination and P3 protocol that is based on immersed in ethanol 70% (1 min), then in hypochlorite sodium at 20% (1,2 p.a) plus tween (20 min), obtaining low fungal contamination (3%), both being favorable for disinfection protocols. In the stage of introducing the MS medium reduced to 25 % of the concentration of salts obtained higher germination percentage (41%). For multiplication was greater number of shoots (3,38) with T2 treatment which consisted of 0 ppm 0,5 ppm of IAA and BAP. And finally during the adaptation to the substrate the best results for variable grafting (81 %). For chocho, an ideal disinfection was achieved on having used the treatment P1, with ethyl alcohol at 96 % (10 sec), followed by sodium hypochlorite at 50 % v/v (10 min), obtaining low percentage of bacterial contamination (2%) and fungal (5%); as for the introduction to the culture medium, the MS diminished to the half of his concentration was chosen on having reached 100 % of germination. In multiplication, the treatment T5 with 1 ppm BAP and 0,5 ppm AIA, achieved a higher average rate of multiplication
(3,22). In the rooting phase higher percentage of presence of roots (64%) was obtained with 1 ppm of ANA combined with 1000 mg of activated charcoal and finally at the stage of adaptation coconut fiber substrate showed higher percentage of grafting (71%) and adaptability (79%). |
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