Aislamiento de taxones de hongos filamentosos capaces de producir amilasas a partir de tubérculos de uso tradicional en la región andina
The industry of processing roots and tubers generated large amounts of starch annually, which disposal is inappropriate. Starch biotansformation into simple carbohydrates is one alternative that added value to a substance listed in first instance as waste. The research objective was to isolate...
| Autor Principal: | Molina Tufiño, Josueth Rafael |
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| Otros Autores: | Sanmartín Ambuludí, Daniel Migoban |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12535 |
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| Sumario: |
The industry of processing roots and tubers generated large amounts of starch
annually, which disposal is inappropriate. Starch biotansformation into simple
carbohydrates is one alternative that added value to a substance listed in first instance
as waste. The research objective was to isolate filamentous fungi that are able to
produced amylase from oca (Oxalis tuberosa) and mashua tubers (Tropaeolum
tuberosum), collected in Saquisilí canton.
Specific YPS medium adding 5% of yucca starch and 5% of potato starch was used.
Eight taxa grouped into 4 genres: Mucor, Penicillium, Fusarium and Trichocladium
were selected. Twenty-eight treatments were established in which growth kinetics
and sugar production was determined in a period of 120 hours.
T11, T5, T16 and T27 treatments were the most productive of total sugars at 110 g/L
of starch. Growth kinetics showed that the treatments had an adaptation phase of less
than 24 hours. It was determined that, the more amount of biomass, the more
production of sugar. We concluded that T5 and T11 were the best treatments, since
they presented a production of 42.1% and 41.5%, respectively. And for this reason,
these treatments are important for future research because they can be applied on a
large scale. |
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