Aislamiento de microorganismos capaces de producir antibióticos, a partir de suelos de las regiones naturales de Ecuador
Acquired resistance by microorganisms is due to the indiscriminate use of antimicrobials and to the ability to originate mechanisms to adapt to different environments. This problem increases in passing years and research of new antibiotics from natural sources being required, their production...
| Autor Principal: | Egas Rosero, Carla Isabel |
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| Otros Autores: | Tinajero Carrera, Michelle Elizabeth |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12142 |
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| Sumario: |
Acquired resistance by microorganisms is due to the indiscriminate use of antimicrobials
and to the ability to originate mechanisms to adapt to different environments. This
problem increases in passing years and research of new antibiotics from natural sources
being required, their production is based on secondary metabolism of microorganisms,
which may come from soils.
The experimental work was focused on the isolation of antibiotic producing strains,
physiological and biochemical characterizations were performed and in which it was
determined that the strains were: Burkholderia cepacia (PAG C007), Burkholderia
cepacia (PAQ C024), Xanthomona maltophilia (PAA C019). Considering pH and
temperature parameters, the optimum conditions in the growth kinetics are 30 °C
temperature and pH 7 ± 0.2. In susceptibility study technique three strains were selected,
and the extracted antibiotic was subjected to infrared spectroscopy, functional groups
found were: amino, carbonyl with double bond lateral chain and alkanes, supporting the
existence of antimicrobial structures. |
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