Identificación molecular de cepas de levaduras con capacidad fermentativa y resistencia alcohólica aisladas de pitahaya (Stenocereus queretaroensis f.a.c Weber).
This work was based on the use of six isolated yeasts from the pitahaya’s fruit (Stenocereus queretaroensis f.a.c Weber) of Coba P. (2013-2014) project CIVABI - UPS, they presented alcohol resistance between 10-12% and medium - high fermentative capacity, for molecular identification; since pr...
| Autor Principal: | Paredes Morales, María Fernanda |
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| Otros Autores: | Yugsi Vargas, Irene Elizabeth |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12145 |
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| Sumario: |
This work was based on the use of six isolated yeasts from the pitahaya’s fruit
(Stenocereus queretaroensis f.a.c Weber) of Coba P. (2013-2014) project CIVABI -
UPS, they presented alcohol resistance between 10-12% and medium - high
fermentative capacity, for molecular identification; since previous biochemical tests
RapID Yeast Plus System had not specificity over their identification due the different
anamorphs states verified later on their subsequent microscopic assays. For the yeast`s
molecular identification, the region ITS was amplified by PCR using primers ITS 1
and ITS 4; the pure strains were sequenced by Sanger, the consortia pass over new
generation sequencing techniques. In the sequences analysis was identified of pure
strains the Pichia kudriavzevii specie with BLAST analysis QC 100% and 100% Ident
and Fungal sp. with 100% QC and 100% Ident. For the interpretation of the consortia
sequences was employed software Geneious R8 (Biomatter Ltd) that allows to obtain
consensus sequences for further BLAST analysis. N010/3/D and N019/3/D consortia
were formed by Saccharomycetales order with QC 99% and Ident 92%; individuals
the Pichia sp. genus with QC 99% and Ident 91%, and Pichia kluyveii specie with QC
100% and Ident 97%. Despite the results obtained, the total number of species that
conform each consortium cannot be assured. |
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