Identificación de microorganismos de suelos de la provincia de Pichincha, con capacidad de producir antibióticos de amplio espectro.
The microorganisms have acquired resistance to various antibiotic substances due to their ability to generate different adaptation mechanisms in stress conditions due to the misuse of antimicrobial substances. Resistance to these substances has increased throughout the world, causing very high morta...
| Autor Principal: | Núñez Reinoso, José Alejandro |
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| Otros Autores: | Sierra Arias, César David |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15186 |
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| Sumario: |
The microorganisms have acquired resistance to various antibiotic substances due to their ability to generate different adaptation mechanisms in stress conditions due to the misuse of antimicrobial substances. Resistance to these substances has increased throughout the world, causing very high mortality rates; that´s why, it is very important to research new antibiotic substances, which can be obtained from secondary metabolites, formed by microorganisms from the soil.
The experimental research was based on the application of bacterial sensitivity techniques, to obtain bacteria capable of producing antibiotic substances, the same ones that were isolated and identified with various biochemical tests and morphological analyzes. It was determined that the only bacteria that showed antimicrobial activity was: Bacillus megaterium (PAP48G3). With this strain, the ability to produce antibiotics was analyzed, under conditions that involve variation in salinity and pH in culture media, against Pseudomonas aeruginosa and Bacillus spizizenii. It was determined that this bacterium produces greater antimicrobial activity against Pseudomonas aeruginosa in a culture broth hypersaline ISP2 (1.2%) and with a range of pH 7 on day 3. This bacterium also produces greater antimicrobial activity against Bacillus spizizenii in a culture broth Hypersaline ISP2 (1.2%) and with a pH 7 range on day 4. |
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