Identificación molecular del género Passiflora (Passifloraceae), de la región norte del ecuador por medio del método DNA Barcoding
The name of the genus Passiflora means flower of passion, on the other hand this genus has a high economic interest due to medicinal, nutritional and ornamental use of its flowers and fruits, in Ecuador there are records of 95 species of this genus, in this research samples of species of this group...
| Autor Principal: | Miño Domínguez, Jorge Eduardo |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/16148 |
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The name of the genus Passiflora means flower of passion, on the other hand this genus has a high economic interest due to medicinal, nutritional and ornamental use of its flowers and fruits, in Ecuador there are records of 95 species of this genus, in this research samples of species of this group present in the northern region of Ecuador were collected at random in the provinces of: Pichincha, Santo Domingo, Napo , Tungurahua, Cotopaxi; in altitudinal floors ranging from 400 to 2800 meters above sea level, the samples were identified at the species level by comparison with vouchers of the National Herbarium of Ecuador QCNE, for the molecular identification the DNA was extracted by the Doyle y Doyle method, then amplified a segment of the matK gene by means of the conventional PCR technique, PCR products were sequenced using the Sanger technique, the sequences were analyzed with the Blast and Boldsystem tools to identify which species they correspond to, Blast being the best tool for identification. Next, a phylogenetic analysis was performed using the segments of the matK gene and the rcbL segment information that is available in Genbank, 3 trees were elaborated one for each segment and one concatenating the trees constructed by the matk segment and the concatenated one show similarity in the grouping of species in accordance with the morphology, while the segment rbcL showed a difference in the distribution. |
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