Análisis de la fragmentación del ADN en el personal de laboratorio de docencia e investigación expuesto a mutágenos químicos en la ciudad de Quito
In Ecuador, the staff working in teaching and research laboratories are exposed to a lot of genotoxic substances, which may cause chromosomal and genetic disorders. However, even those that may be causing damage to DNA due to constant contact with these chemicals are unknown. The aim of this study w...
| Autor Principal: | Bonilla Jerez, Adriana Melisa |
|---|---|
| Otros Autores: | González Cano, Diana Abigail |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/9410 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: |
In Ecuador, the staff working in teaching and research laboratories are exposed to a lot of genotoxic substances, which may cause chromosomal and genetic disorders. However, even those that may be causing damage to DNA due to constant contact with these chemicals are unknown. The aim of this study was to evaluate DNA fragmentation volunteer individuals working in labs for teaching and research in Quito that are exposed to chemicals and the relationship with risk factors such as chemical compound, exposure time and personal protective equipment. To study peripheral blood samples of 30 individuals occupationally exposed and 10 control subjects were taken, the comet assay technique was performed and by visual record 200 Nucleoids analyzed by individual, determining the level of fragmentation and classifying at zero, low, medium tall and full. The results indicate that exposure to mutagenic chemicals is related to DNA fragmentation, exhibiting a significant difference in the zero and secondary levels (p = 0.000), low (p = 0.002) (p = 0.012), between the exposed group and control.
Although there are no significant differences regarding the type of chemical compound, individuals who have high levels of fragmentation were found exposed to organic solvents and have more than 6 years’ time exposure. |
|---|