Evaluación de la actividad antioxidante bioautográfica de 5 variedades de aceites esenciales amazónicos (Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum; Piper imperiale)

This experimental study evaluated the antioxidant potential of five Amazon species collected at the Kutuku biological station at the Universidad Politécnica Salesiana, (Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum y Piper imperiale), all they used by locals for their medici...

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Autor Principal: Chasipanta Ronquillo, Eliana Cristina
Otros Autores: Chicaiza Galarza, Tatiana Silvana
Formato: bachelorThesis
Idioma: spa
Publicado: 2016
Materias:
Acceso en línea: http://dspace.ups.edu.ec/handle/123456789/12141
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Sumario: This experimental study evaluated the antioxidant potential of five Amazon species collected at the Kutuku biological station at the Universidad Politécnica Salesiana, (Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum y Piper imperiale), all they used by locals for their medicinal properties. In order to quantify the radical scavenging capacity of the essential oils the DPPH and ABTS spectrophotometric methods were used, providing the following results respectively: P. auritum (4,931; 2,961); O. quixos (11,291; 7,897), E. stipitata (12,868; 18,834), P. guajava (89,788; 50,155) and P. imperiale (313,724; 179,383), at a 50% (IC50) inhibitory concentration of oxidation in ul/mL. The antioxidant property was determined by the β-carotene bleaching test, which took the mean value from IC50 in ul/mL and provided the following values: P. auritum (3, 19E-02), O. quixos (4, 28E 01), P. guajava (2, 15E-01) and P. imperiale (4, 82E+01). The natural essential oil of Thymus vulgaris was used as a positive control. The essential oils that present the best antioxidant properties in order of strength were P. auritum, O. quixos y E. Stipitata, and in a lesser degree P. guajava and P. imperial. The bioautographic assays revealed discoloration for P. auritum with safrole as responsible for this activity, E. stipitata with δ -cadidene as responsable and in O. quixos the active components were caryophyllene, caryophyllene oxide, α-humulene and α-copaene.