Evaluación de la actividad antioxidante bioautográfica de 5 variedades de aceites esenciales amazónicos (Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum; Piper imperiale)
This experimental study evaluated the antioxidant potential of five Amazon species collected at the Kutuku biological station at the Universidad Politécnica Salesiana, (Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum y Piper imperiale), all they used by locals for their medici...
Autor Principal: | Chasipanta Ronquillo, Eliana Cristina |
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Otros Autores: | Chicaiza Galarza, Tatiana Silvana |
Formato: | bachelorThesis |
Idioma: | spa |
Publicado: |
2016
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Materias: | |
Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/12141 |
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Sumario: |
This experimental study evaluated the antioxidant potential of five Amazon species
collected at the Kutuku biological station at the Universidad Politécnica Salesiana,
(Ocotea quixos; Psidium guajava; Eugenia stipitata; Piper auritum y Piper
imperiale), all they used by locals for their medicinal properties.
In order to quantify the radical scavenging capacity of the essential oils the DPPH and
ABTS spectrophotometric methods were used, providing the following results
respectively: P. auritum (4,931; 2,961); O. quixos (11,291; 7,897), E. stipitata (12,868;
18,834), P. guajava (89,788; 50,155) and P. imperiale (313,724; 179,383), at a 50%
(IC50) inhibitory concentration of oxidation in ul/mL. The antioxidant property was
determined by the β-carotene bleaching test, which took the mean value from IC50 in
ul/mL and provided the following values: P. auritum (3, 19E-02), O. quixos (4, 28E
01), P. guajava (2, 15E-01) and P. imperiale (4, 82E+01). The natural essential oil of
Thymus vulgaris was used as a positive control. The essential oils that present the best
antioxidant properties in order of strength were P. auritum, O. quixos y E. Stipitata,
and in a lesser degree P. guajava and P. imperial. The bioautographic assays revealed
discoloration for P. auritum with safrole as responsible for this activity, E. stipitata
with δ -cadidene as responsable and in O. quixos the active components were
caryophyllene, caryophyllene oxide, α-humulene and α-copaene. |
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