Estandarización fitoquímica de extractos hidroalcohólicos de Ishpingo, Ocotea quixos (Lam.) Koste.

The phytochemical standardization of hydroalcoholic extracts of Ishpingo Ocotea quixos Lam. KOSTERN consisted in evaluating the influence of three experimental variables: plant material (fresh and dry), solvent (ethanol and cane ethanol) and solvent concentration (90, 70, 50) % v/v through an...

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Autor Principal: Flor Olivo, Héctor Ivan
Otros Autores: Parra Pedraza, Michelle Jazmín
Formato: bachelorThesis
Idioma: spa
Publicado: 2017
Materias:
Acceso en línea: http://dspace.ups.edu.ec/handle/123456789/14166
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Sumario: The phytochemical standardization of hydroalcoholic extracts of Ishpingo Ocotea quixos Lam. KOSTERN consisted in evaluating the influence of three experimental variables: plant material (fresh and dry), solvent (ethanol and cane ethanol) and solvent concentration (90, 70, 50) % v/v through an experimental factorial design which one we obtained 12 different extracts with 3 repetitions of each of them. A phytochemical screening showed the presence of phenols, tannins, catechins, saponins, quinones, coumarins, lactones and alkaloids in the 36 extracts; we used the Folin - Ciocalteu method in order to quantitatively determine the total phenol concentration and by statistical analysis ANOVA (Tukey 95%) proves that there is significant difference between the 12 established treatments where the extracts with the mayor quantity of phenols are AMF50:50 (Alcohol, Fresh Material, Concentration 50% v/v) and CMF90:10 (Cane alcohol, Fresh Material, Concentration 90% v/v) with 12,538 and 13,298 mg of gallic acid / mL of extract respectively; concluding that, this last combination allows to obtain greater quantity of total phenols following the established standardized protocol.