Estandarización fitoquímica de extractos hidroalcohólicos de Ishpingo, Ocotea quixos (Lam.) Koste.
The phytochemical standardization of hydroalcoholic extracts of Ishpingo Ocotea quixos Lam. KOSTERN consisted in evaluating the influence of three experimental variables: plant material (fresh and dry), solvent (ethanol and cane ethanol) and solvent concentration (90, 70, 50) % v/v through an...
Autor Principal: | Flor Olivo, Héctor Ivan |
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Otros Autores: | Parra Pedraza, Michelle Jazmín |
Formato: | bachelorThesis |
Idioma: | spa |
Publicado: |
2017
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Materias: | |
Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/14166 |
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Sumario: |
The phytochemical standardization of hydroalcoholic extracts of Ishpingo Ocotea
quixos Lam. KOSTERN consisted in evaluating the influence of three experimental
variables: plant material (fresh and dry), solvent (ethanol and cane ethanol) and solvent
concentration (90, 70, 50) % v/v through an experimental factorial design which one
we obtained 12 different extracts with 3 repetitions of each of them. A phytochemical
screening showed the presence of phenols, tannins, catechins, saponins, quinones,
coumarins, lactones and alkaloids in the 36 extracts; we used the Folin - Ciocalteu
method in order to quantitatively determine the total phenol concentration and by
statistical analysis ANOVA (Tukey 95%) proves that there is significant difference
between the 12 established treatments where the extracts with the mayor quantity of
phenols are AMF50:50 (Alcohol, Fresh Material, Concentration 50% v/v) and
CMF90:10 (Cane alcohol, Fresh Material, Concentration 90% v/v) with 12,538 and
13,298 mg of gallic acid / mL of extract respectively; concluding that, this last
combination allows to obtain greater quantity of total phenols following the
established standardized protocol. |
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