Identificación molecular de consorcios bacterianos resistentes a metales pesados provenientes de aguas residuales de una industria hidrocarburífera en la provincia de Esmeraldas-Ecuador
In this paper resistant bacterial consortia to metals were molecularly identified, isolated from wastewater of a hydrocarbon industry in the province of Esmeraldas. Samples were inoculated in four culture media and metal susceptibility testing was performed considering TULAS`s maximum dischar...
| Autor Principal: | Anguisaca Arias, Cristian Gabriel |
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| Otros Autores: | Castañeda González, Edgar Andrés |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13241 |
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| Sumario: |
In this paper resistant bacterial consortia to metals were molecularly identified, isolated
from wastewater of a hydrocarbon industry in the province of Esmeraldas. Samples were
inoculated in four culture media and metal susceptibility testing was performed
considering TULAS`s maximum discharge limits: cadmium (0.2, 2 and 20 ppm),
chromium (2, 25 and 50 ppm) and lead (2, 10 and 20 ppm). Metal removal rate was
evaluated by spectrophotometric for cadmium, chromium and lead and arsenic removal
by Lovibond kit Arsenic Test (highly sensitive) No. 400700. Five consortiums were
isolated, where three showed a significant percentage of metals removing activity:
ReO3As1 (20.78%), ReO6Cr1 (2) A (21%) and Re04Pb2 (1) A (22.49%). For molecular
identification DNA was extracted using the method proposed by Ausubel, then the
samples were sent to Macrogen-Korea, where the sequencing of new generation was
performed, obtaining genomic libraries of fragments of the region of the 16S rRNA gene,
amplicons were generated with a size of 600 bp and the sequencing was performed with
the GS FLX Titanium equipment. The pyrograms formed were analyzed using the Roche
GS FLX software (v 3.0) program. The identification was performed with the BLAST
program. The three consortiums submitted belonging individuals to the genus
Pseudomonas (Query Cover 99% and Ident 98%) and Staphylococcus (Query Cover
100% and Ident 99%). There was not possible identify species. These bacterial genera
found have previously proved their effectiveness in environmental bioremediation
processes. |
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