Identificación molecular de bacterias resistentes a cadmio y cromo aisladas de aguas contaminadas de la zona de Papallacta cantón Quijos de la provincia de Napo
Cadmium and Chromium are highly toxic heavy metals in their ionic forms, they’re extremely hard to remove because they tend to accumulate becoming a serious environmental problem. The bacterial ability to resist high concentrations of heavy metals was been studied in the bioremediation field,...
| Autor Principal: | Bustos Viteri, Krupskaya Tamara |
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| Otros Autores: | Cacuango Gualacata, Jenny Jacqueline |
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13535 |
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| Sumario: |
Cadmium and Chromium are highly toxic heavy metals in their ionic forms, they’re
extremely hard to remove because they tend to accumulate becoming a serious
environmental problem. The bacterial ability to resist high concentrations of heavy metals
was been studied in the bioremediation field, the genetic information in bacterial genome
contain sections that encode mechanisms that allow them to survive and adapt to polluted
environments.
To identify Cd and Cr resistant bacteria, bacterial consortia were cultured in three growth
medium (PCA, Cetrimide agar and culture broth) at two temperatures (4°C and 37°C) and
two incubation times (24 and 48 hours), bacterial sensitivity was evaluated at 2 and 20
ppm Cd, and 50 and 500 ppm Cr concentrations.
The growth medium (Cetrimide agar and culture broth) and the incubation time were
determined to influence significantly the bacterial growth through the application of
generalized linear models, bayesian information criteria and analysis of variance.
Sensitivity tests showed that bacterial consortia are more sensitive to 2 and 20 ppm Cd
concentrations.
Finally, the DNA from bacterial consortia that showed resistance in the sensitivity assays
was extracted, the 16s rRNA marker was amplified and sequenced, through the sequences
alignment it could be possible identified Cd and Cr resistant bacteria mainly correspond
to the genus Pseudomonas, Staphylococcus and Stenotrophomonas |
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