Evaluación de la biodegradación de un insecticida carbamato en muestras de suelo de cultivo de papa, mediante Trichoderma harzianum y Pleurotus ostreatus.
his research aims to evaluate the biodegradation of the carbofuran insecticide by bioremediation of the fungi Trichoderma harzianum and Pleurotus ostreatus in soil samples from potato cultivation. The methodological procedure was carried out in two phases: the field phase consisted of georeferencing...
| Autor Principal: | Paredes Sandoval, Michelle Katherine |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13842 |
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| Sumario: |
his research aims to evaluate the biodegradation of the carbofuran insecticide by bioremediation of the fungi Trichoderma harzianum and Pleurotus ostreatus in soil samples from potato cultivation. The methodological procedure was carried out in two phases: the field phase consisted of georeferencing and soil sampling of the potato crop in "El Capulí" Carchi; the sampling of Pleurotus ostreatus was in Calacalí, in addition an aquarium was acclimatized for its growth. The laboratory phase consisted in the physicochemical analysis at the beginning and end of the project of soil nutrients, pesticides by HPLC; the isolation of T. harzianum based on the soil of potato cultivation and the isolation of P. ostreatus from fungal fruiting (cap); were obtained by microbiological techniques. A randomized complete design (DCA) was applied with 4 replicates, 4 treatments and 16 experimental units. The mentioned treatments were T0 (Soil with insecticide), T1 (Soil with insecticide and Trichoderma harzianum), T2 (Soil with insecticide and Pleurotus ostreatus) and T3 (Soil with insecticide plus Trichoderma harzianum and Pleurotus ostreatus). The T1 treatment, whose Trichoderma inoculum concentration used was 38.3x106 conidia/mL solution; proved to be the best with 63% biodegradation corresponding to 4170.5 μg/g of carbofuran in 57 days. Thus the bioaumentation of conidia presents an alternative to biodegrade recalcitrant molecules, difficult to break by physical-chemical processes. This biological technique presents an option because the economy and the environmental impact are low. |
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