Evaluación in vivo de la capacidad antagónica de Lactobacillus acidophilus frente a Propionibacterium acnes en jóvenes con diagnóstico previo de acné tipo II, Parroquia de Zámbiza, Distrito Metropolitano de Quito.
This study aimed to evaluate the in vivo antagonistic effect of Lactobacillus acidophilus (L.acidophilus) against Propionibacterium acnes (P. acnes) the bacteria causing acne, by formulating a lotion. To carry out this investigation, first evaluating the antagonistic effect in vitro of L. acidophil...
| Autor Principal: | Pumisacho Ocaña, Victoria Sheila |
|---|---|
| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2015
|
| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/8167 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: |
This study aimed to evaluate the in vivo antagonistic effect of Lactobacillus acidophilus (L.acidophilus) against Propionibacterium acnes (P. acnes) the bacteria causing acne, by formulating a lotion.
To carry out this investigation, first evaluating the antagonistic effect in vitro of L. acidophilus and four lotion with L.acidophilus was performed using the Anukool method, by measuring the halos of inhibition obtained and by analyzing one-way nonparametric Kruskal-Wallis test and a posteriori (All-Pairwise Comparisons test) in the Statistix 8.0 software, variance results have revealed that 1x 108 cfu/ml concentration is obtained in which L. acidophilus inhibits the growth of P. acnes. The analysis also found that the lotion A, was the formulation showed higher inhibition in vitro being suitable for use in the in vivo study, this formulation was subjected to a test of irritability (Patch Test only Simple) resulting in an index primary skin irritation 0.19 that qualifies as "well tolerated", indicating that does not cause skin irritation and can be safely used in the study of cosmetic activity in vivo.
To study cosmetic activity in vivo non-invasive method used exploration using bioengineering instrument Visiopor PP34N®, which quantifies the porphyrins in the skin. Through a mathematical analysis it was determined that there was a decrease of porphyrins during treatment indicating that the lotion has an antagonistic effect against P. acnes and can be used reliably in the treatment of this disease. |
|---|