Actividad alexítera DE Adenostemma lavenia L. (KUNTZE) Y Pollalesta discolor (KUNT) aristeg. sobre el veneno de Bothrops atrox (PITALALA).
Ecuador holds one of the biggest diversity of reptiles in the world, thus, there is a huge number of venomous snakes; there are specimens of the genre Bothrops in the Amazon, being B. atrox the main cause of snake attacks in the area. Many indigenous communities treat this kind of accident by using...
| Autor Principal: | Almeida Vaca, Daniela Belén |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/15658 |
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| Sumario: |
Ecuador holds one of the biggest diversity of reptiles in the world, thus, there is a huge number of venomous snakes; there are specimens of the genre Bothrops in the Amazon, being B. atrox the main cause of snake attacks in the area. Many indigenous communities treat this kind of accident by using alcoholic and heptanic extracts of vegetable species with anihemolytic and antiproteolytic activities. This project’s objective was to analyze the antidote activity of two Amazonian vegetable species, Adenostemma lavenia and Pollalesta discolor, respectively known as Araratz and Pigue, against the venom of Bothrops atrox. In vitro techniques were used to analyze anticoagulant, antihemolytic and antiproteolytic activities of the extracts over human blood and its components; changes in venom’s proteic profile were observed through SDS-PAGE, before and after its exposure to both extracts. The obtained results reveal that both species have neutralizing activity over the venom’s hemotoxic capabilities; but in comparison with alcoholic and heptanic extracts of A. Lavenia, the alcoholic extract of P. discolor is the best inhibitor in anticoagulant, antihemolytic and antiproteolytic activities; furthermore, the electrophoretic profiling, showed that the number of protein bands of the poison increased under the effects of both species, being the extract of P. discolor the one that presented the biggest amount of bands; this concludes that the enzymatic activity of the snake’s poison is diminished by organic components found in both vegetable species. |
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