Inhibición de la actividad hemolítica del veneno de Bothrops atrox por los extractos de Minthostachis cf. mollis (Kunt) Griseb y Pollalesta discolor (Kunt) Aristeg.
Ethnobotanical knowledge is important because some plant species are used to counteract the effects of the biting of poison snakes. In this research we tested the antihemolytic activity of the species Minthostachys cf. Mollis (Kunth) Griseb (kurarina) and Pollalesta discolor (Kunth) Aristeg (pigu...
| Autor Principal: | Muñoz Carrión, Ronalt Cristian |
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| Formato: | bachelorThesis |
| Idioma: | spa |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: |
http://dspace.ups.edu.ec/handle/123456789/13642 |
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| Sumario: |
Ethnobotanical knowledge is important because some plant species are used to
counteract the effects of the biting of poison snakes. In this research we tested the antihemolytic
activity of the species Minthostachys cf. Mollis (Kunth) Griseb (kurarina)
and Pollalesta discolor (Kunth) Aristeg (pigue), species that are widely used by
Amazonian indigenous inhabitants to counteract the effects of poisson of snake
Bothrops atrox (pitalala), a species frequently responsible for Ophidian accidents,
considered today as a public health problem. The plant samples of these species were
collected in the province of Morona Santiago, in the Morona and Huamboya cantons.
Total dry extracts were prepared with increasing polarity solvents (n-heptane, ethanol
and water) in order to investigate their qualitative and quantitative chemical
composition; the quantification of total phenols and flavonoids were performed by
visible light with spectrophotometry. Their inhibition haemolysis caused by pitalala
venom using blood agar medium phosphatidylcholine was investigated in vitro. The
results showed that the alcoholic extracts of both plants have a higher antihemolytic
activity than the other extracts, established by their ability to decrease the haemolysis
halo of a dose of poison of 15 μg. |
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